Intelligent Primer Design Platform

批量设计跨外显子连接的qPCR引物，支持多物种，自动选择 canonical transcript。

Workflow Batch Gene Panel

Output Excel / CSV / Order Sheets

Focus Specificity-first primer ranking

Design Console Assay Setup

Mode Batch Design

Stack RefSeq / Primer3

Exon-aware workflows with multi-primer export paths for direct ordering.

01Input Genes

02Tune Constraints

03Review and Export

Batch-ready qPCR primer design with direct ordering handoff.

English-first controls, Chinese helper notes. Use this surface to design exon-aware primers, compare candidate pairs, and export order-ready sheets.

Gene Input

Start with one gene symbol per line. 输入基因名后即可批量设计。

Gene Symbols 基因名列表

Paste from Excel or enter manually

Species 物种

Design Parameters

Keep defaults for routine assays, or narrow the range if you already know the amplification window. 默认参数适合大多数 qPCR 场景。

Product Size (bp)

—

Primer Length (bp)

—

Melting Temp (°C)

—

GC Content (%)

—

Primer pairs per gene

Prioritize exon junction spanning primers. 优先跨 exon junction。

Outputs include ranked primer pairs, Excel export, CSV export, and cumulative ordering sheets.

ΔΔCt Method

qPCR Data Analysis Platform

分析 qPCR 实验数据，统一导入、分组、绘图和导出流程，保留手动修正空间。

Import Bio-Rad / Manual / CSV

Analysis ΔΔCt + Optional plot annotation

Export Result tables and publication plots

Analysis Console Review Surface

Input Mapped Ct Rows

Output Table / PNG / PDF

Stage import, normalization, plotting and export in one continuous analysis workspace.

01Analysis Settings

02Data Input

03Analyze

04Plot and Export

Data Input

Choose Bio-Rad import for plate-oriented review, or switch to a direct Ct table if your data is already tidy. Bio-Rad 流程适合先看孔板再分析。

Bio-Rad CFX Import

Upload a CFX export, map wells, then generate analysis-ready Ct rows. 上传 CFX CSV 后可自动映射孔位。

Step 1 — Upload Bio-Rad CFX Export CSV

Choose File No file chosen

CFX Maestro → Export → Quantification Data → CSV · 上传后 Cq 值自动显示在孔板上

Step 2 — Annotate Wells (Sample / Gene / Rep)

Quick Tags 选中孔后点击可直接填充；未选孔时仅写入上方输入框

Replicate 自动分配：同一样本的重复块按孔位顺序编号 R1/R2/R3；同一块里的不同基因共享同一个 R

拖拽可分批累计框选多个区域；从已选孔开始拖拽可取消选中，再用 Quick Fill 或 Batch Fill Series 批量标注

Analysis Settings

Reference Gene (Housekeeping) 内参基因

Control Group 对照组

Plot Mode 绘图模式

Auto Auto-detect log2FC / relative expression Induction Log2 fold change (best for high expression) Knockdown Relative expression % (best for low expression)

Automatic ΔΔCt calculation with fold-change output. 自动完成 ΔΔCt 与倍数变化计算。

Analysis returns gene-level summaries, exportable tables, and plot-ready data for PNG or PDF output.

Analysis Results

Generate Plot

Plot Settings 修改后自动刷新预览

Preset

Style Preset

Statistical Annotation

Font Size

Label Angle

Bar Width

Group Gap

Dot Size

Dot Spread

Figure Width

Figure Height

Y Max

Legend

Axis Line Width

Marker Line Width

Tick Density

Y Tick Step

Reference Line

Grid

Top Frame

Right Frame

统计分析仍在 Analyze Data 阶段默认计算；Plot Settings 里的 Statistical Annotation 只控制图上是否显示显著性标注

Community Verified

Community Primer Library

Browse user-contributed, system-validated qPCR primer pairs, or contribute your own verified primers.

Validation Transcript alignment + amplicon check

Scope Human, Mouse, Rat, Zebrafish, Fly

Contribute Submit your verified primers

Community Stats Primer Library

Total Primers --

Genes Covered --

📄 Download pre-designed primer set

Browse & Filter

Species

Gene

Sort

Species

Gene Symbol

Forward Primer (5'→3')

Reverse Primer (5'→3')

Notes (optional)

Upload CSV File

📥 Download template CSV — fill in your primers, then upload below.

Choose CSV File No file chosen

Row	Gene	Result	Size	Transcript