CRISPR Guide Workbench
Library-backed and de novo sgRNA selection for human and mouse targets, including direct sequence and FASTA-based design.
Use library mode for fast retrieval, or switch to design mode for sequence-driven guide discovery.
Human-readable defaults first, with deeper transcript and guide-map detail only when you expand a gene card.
Target Input
Start from gene symbols for the fastest path, or switch to pasted sequence / FASTA when you already know the exact target region.
Choose whether to design from gene symbols, a pasted DNA sequence, or uploaded FASTA records.
Whitespace and line breaks are ignored. Sequence-derived guides use input-relative coordinates and do not include genome-backed transcript mapping.
Upload one FASTA file containing one or more records.
Selection Setup
Pick retrieval mode, set species context, then request only the number of guides you actually want to review.
Library-backed selection for human Brunello, mouse Bassik, and fruit fly Perrimon guides.